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SEMINAR: Bayliss Seminar Series

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Today's date is Saturday, February 27, 2021
Bayliss Seminar Series : Deciphering RNA recognition by Pentatricopeptide Repeat Proteins in Arabidopsis Other events...
PPR proteins are modular RNA-binding proteins with repeated 35-amino acid sequence motifs that control mitochondrial and plastid gene expression. They are being considered as a potential tool for manipulating gene expression in plants because they can recognize a wide range of different RNA sequences and the molecular basis for this sequence recognition is partially known and understood, allowing their specificity to be deliberately altered. Each PPR motif recognizes and binds a single nucleotide with a 1:1 correspondence between contiguous protein motifs and RNA bases in the target RNA. This recognition is determined primarily by hydrogen bonding patterns between the RNA base and two amino acid side chains at specific positions in each PPR motif. Modifying these amino acids should alter target specificity. This project is designed to provide insights about RNA recognition by PPR proteins and the possibility of redirecting them to desired targets. The project will focus on generating a PPR library sequence that can bind to many different targets. The intention is to create a large number of variants of PPR proteins and test if they bind RNA with the expected specificity in vitro. Finally, I will express these proteins in plants and characterize their effects on mitochondrial gene expression, with a view to potential applications in plant breeding. These experiments should expand our knowledge of RNA specificity and binding by PPR proteins in plant mitochondria and contribute to the ultimate goal of developing specific RNA targeting tools using PPR proteins that can be aimed at desired targets.
Speaker(s) Lilian Vincis Pereira Sanglard, School of Chemistry and Biochemistry
Location Bayliss Lecture Theatre G:33
Contact <[email protected]>
Start Thu, 24 Nov 2016 12:00
End Thu, 24 Nov 2016 13:00
Submitted by scbevents <[email protected]>
Last Updated Wed, 26 Oct 2016 12:56
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