SEMINAR: CMCA Seminar Series: Cryo-SEM
|CMCA Seminar Series: Cryo-SEM : A cool technique for imaging hydrated and beam sensitive specimens in their natural state
Following a successful 2011 ARC LEIF bid, CMCA will acquire cryo-SEM capabilities this coming year, to provide improved imaging capabililties for hydrated and beam sensitive samples. Marilyn Carey from Gatan UK will present information on cryo-SEM techniques for those wanting to know more about cryo-SEM or SEM in general, illustrated with some fantastic images.
The high vacuum present in a SEM is a highly invasive environment for hydrated, low melting point and volatile specimens. Specimens of this type when placed into the SEM chamber readily collapse, providing little or no cryo-fixation. Such samples remain as close as possible to their natural state in the high vacuum of the SEM and allow their examination under the electron beam.
In addition, cryo-fixed samples can be fractured to expose internal microstructure and etched to enhance micro-structural detail, providing further information. To improve resolution further, samples may also be coated prior to imaging. The technique can be applied to a large range of samples with regards to both biological and materials applications.
The technique is simple and quick to undertake and provides high resolution data of value.
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