SEMINAR: Programmable RNA recognition by CRISPR/Cas9
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Mitchell received his PhD in Biochemistry from the University of Sydney in 2013, where he worked in Prof. Joel Mackay's group to develop artificial zinc-finger proteins to specifically recognize RNA for sequence-specific RNA-targeting applications. In 2013, Mitchell moved to the University of California, Berkeley to take up a postdoctoral position in the lab of Prof. Jennifer Doudna. In the Doudna lab, Mitchell has focused on exploring the molecular mechanisms of RNA-mediated gene regulation and the development of tools to interrogate these processes. In early 2012, the Doudna laboratory was the first to show the programmable, sequence-specific DNA recognition and cleavage ability of a CRISPR-associated protein known as Cas9. This research led to an explosion in the use of CRISPR/Cas9 as a genome-editing tool in multiple cell types and organisms. However, this protein had been thought to be incapable of targeting RNA. In a recent publication in Nature, Mitchell and colleagues were able to show that this is not the case and Cas9 can be used as a readily programmable sequence-specific RNA-binding and cleavage enzyme. This discovery paves the way for sequence-specific tools to explore the myriad of RNA species implicated in gene regulation and disease. Mitchell is now building on his work by exploring a number of applications of CRISPR/Cas9 for untagged RNA transcript analysis, detection and manipulation.
Speaker(s) |
Dr Mitchell O'Connell
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Location |
McCusker Auditorium, Harry Perkins Institute of Medical Research, SCGH, 6 Verdun Street, Nedlands WA 6009
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Contact |
Fiona Mackenzie
<[email protected]>
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URL |
http://www.perkins.org.au
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Start |
Thu, 08 Oct 2015 12:00
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End |
Thu, 08 Oct 2015 13:00
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Submitted by |
Fiona Mackenzie <[email protected]>
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Last Updated |
Mon, 21 Sep 2015 10:04
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